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The md5 module implements the MD5 message digest algorithm (developed by MIT and RSA Data Security, Inc.) to generate 128-bit message digests for arbitrarily long strings of bytes. Create a new md5 object by calling the new([msg]) function. You then repeatedly call the object s update(msg) method until you have passed it all your data. At any time, you can call the digest() method to get the md5 checksum at that point in time:

# A personal Memory-Only database. urlid mem url jdbc:hsqldb:mem:memdbid username sa password # This is for a hsqldb Server running with default settings on your local # computer (and for which you have not changed the password for sa ). urlid pix-sa url jdbc:hsqldb:hsql://localhost/pix username sa password

4. To verify your link between label and option, click the Show button in the Inspector. While this button is pressed, the link appears as shown in Figure 16-8.

4. At the Specify a point or [Hor/Ver/Ang/Bisect/Offset]: prompt, choose point 1, shown in Figure 6-3. 5. At the Specify through point: prompt, choose point 2, shown in Figure 6-3.

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To obtain useful information from ow cytometry, it is essential that cells can be seen by the detector. Detection can take advantage of intrinsic properties of the cell or exploit interactions between cells and extrinsic reagents. Selective information is obtained by controlling the wavelength and other characteristics of the laser light incident on the cells combined with variation in the angle of detection and use of appropriate lters. Modern ow cytometers have the potential for simultaneous detection of scattered light at several wavelengths and angles in individual channels. Intrinsic properties useful in ow cytometry include refractive index and absorption/ uorescence properties of biomolecules within cells. As mentioned above, refractive index measurements quantify granularicity within the cells. Different subpopulations of cells would be expected to have altered granularicity as a consequence of changes in the relative amount and activity of cell organelles. Subtle differences between cell subpopulations can therefore be detected in this way. Absorbance measurements may be used to quantify chromophores such as nucleic acids and haem-containing proteins essentially as described in 3. Useful intrinsic uors include pyridine and avin nucleotides and measurement of these, for example, may be used to detect differences in redox status between different cell subpopulations by measuring NAD: NADH + H+ ratios. Speci c groups of naturally-occurring chromophores such as porphyrins and chlorophylls may also be useful in the study of particular cell types.

Load/Save: You can load and save settings to disk using these buttons. Auto: Click the Auto button to automatically map the darkest pixel in your selection to black and the lightest pixel to white, as if you had chosen Image Adjust Auto Levels. Photoshop actually darkens and lightens the image by an extra half a percent just in case the darkest and lightest pixels are statistically inconsistent with the rest of the image. To enter a percentage of your own, Alt-click the Auto button (the button name changes to Options). This displays two additional options, Black Clip and White Clip. Enter higher values to increase the number of pixels mapped to black and white; decrease the values to lessen the effect. Figure 17-20 compares the effect of the default 0.50 percent values to higher values of 2.50 and 9.99 percent. As you can see, raising the Clip value produces higher contrast effects.

Figure 3.41. Physical basis of infrared spectroscopy. When infrared light of an appropriate frequency is absorbed, the molecule may be promoted through an energy increment E to the rst vibrational energy level which will have a different value for either bond length or bond angle. This is called the fundamental absorption. Different bond types have individual vibrational energy levels which has the consequence that the infrared absorption spectrum is characteristic for a speci c chemical structure.

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