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The default access mode in beacon-enabled operation is slotted CSMA/CA, with some slots optionally reserved for certain nodes During the inactive portion of the superframe, any device may enter a low power mode; the cluster coordinator can switch to the upper cluster in order to perform the bridging function that is, deliver the data to the coordinator of the upper cluster All lower cluster coordinators use this facility to perform the bridging function As soon as the active part of the superframe is completed in the lower cluster, the coordinator/bridge switches to the upper cluster and waits for the beacon so that it can deliver the data from the lower cluster to the upper cluster coordinator/network sink All clusters use the CSMA/CA access, which means that the bridge has to compete for medium access with ordinary nodes in the upper cluster.

Americans traveled abroad for advanced education at British and continental universities after the Civil War; painters, writers, and scientists went in large numbers With the postwar establishment of the new land-grant universities, professional opportunities arose for faculty members, especially in the sciences, for education not yet available in the United States With the zeal of converts and crusaders, the rst generation of North American psychologists returned from their study abroad to stimulate the development of graduate education within established American colleges and universities and the newer land-grant universities (Kohler, 1990) They wrote textbooks to incorporate the results of the continental laboratories, developed courses for undergraduate and graduate students, created laboratories for teaching and research, and founded journals for the publication of research from the newly established laboratories.

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The laboratories came to be the locus of education in psychology in universities and colleges (Calkins, 1910; Sanford, 1910) and came to symbolize psychology as science, while psychology, lodged within departments of philosophy, became the introductory course required for further study in philosophy (Fuchs, 2000b) William James and Evolutionary Theory The essential break with the mental philosophical past was achieved by William James, whose Principles of Psychology (James, 1890) represented the rst of the modern textbooks (Evans, 1981) James was a transitional gure, with one foot in philosophy and the other in the empiricism of the new science His text, while still too philosophical for some of his more empirical colleagues (see, eg, Evans, 1981; Ross, 1972), nevertheless effectively cut the discipline s past ties.

Individuals who become highly anxious during test situations, and consequently perform at a suboptimal level, are thought to divide their attention between task-relevant and task-irrelevant thoughts more so than those who do not become overly anxious in high-pressure situations (Wine, 1971) Additional support for a distraction account of choking comes from recent work specifically examining the impact of performance pressure on cognitive task performance Beilock, Kulp, et al (2004) had individuals perform easy math problems as well as difficult problems (that placed heavy demands on working memory) in both lowand high-pressure situations It was found that pressure does indeed cause individuals to worry Moreover, only those math problems that were strongly reliant on the working memory resources that worries are thought to consume (ie, the difficult problems) showed signs of failure under pressure.

As soon as the data is delivered, the bridge can return to its own cluster This also means that, should the bridge be unable to transmit its data when the (active portion.

4.1. Immunostaining Followed by Cell Fluorescence-Activated Cell Sorting (FACS) Individual or combinations of various membrane-bound proteins can be used to distinguish different cell types from each other. Thus labeling cells with antibodies that are speci c for particular surface proteins and then sorting the desired cells from the population is an approach that may be used for selecting desired cell types. FACS is readily used to isolate such distinct populations of cells at a rapid and reproducible rate [Eiges et al., 2001]. In this technique, cell surface markers are labeled with uorescent antibodies. With a FACS ow sorter, the positive population can then be puri ed from a heterogeneous mixture of cells. The advantage of using FACS is the ability to use a combination of markers, each with a distinctive uorescent label, for a multiparametric sort. Thus cells that coexpress three or four distinguishing proteins can be labeled and isolated, providing a robust method of isolating desired cells. This approach has been used clinically for characterizing and isolating bone marrow cells [Jurecic et al., 1993; Katayama et al., 1993]. In theory, if cell surface markers that de ne any cell population are known, that population can be isolated from developing EBs, making FACS a potentially powerful technique. Despite this power there are several practical limitations of using FACS for cell isolation. For example, a distinctive set of cell surface markers may not be known, or even exist, for a desired cell type. In addition, internal markers such as proteins that reside within the cell cannot be used. Currently, cell permeabilization

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